The film revolves around a special FBI organization of trained secret agent animals, equipped with advanced tools that allows the mammalian members to talk to humans. The primary field team consists of Darwin (Sam Rockwell) (team leader), Juarez (Penelope Cruz) (martial arts), Blaster (Tracy Morgan) (weapons/transportation), star-nosed mole Speckles (Nicolas Cage) (cyber intelligence), and fly Mooch (reconnaissance) (Dee Bradley Baker). The unit's leader, Ben (Zach Galifinakis), orders an unauthorized infiltration of the residence of home electronics and appliances magnate, Leonard Saber (Bill Nighy) - Owner of Saberline Technology, who has been under FBI investigation. The team is able to retrieve sensitive information about a sinister scheme that is set to occur in 29 hours. However, when Ben's superior arrives for his evaluation, he is angry at Ben's unauthorized mission and the fact that the downloaded intelligence appears to be useless information about Saber's coffee makers. As a result, the government agent orders the unit shut down, the equipment seized and the animals to be used as experimental subjects to be killed. With the help of their human compatriots, Darwin, Juarez, Blaster, Mooch, and Speckles escape with hopes of stopping Saber's scheme, but find themselves in a pet carrying case bound for a pet shop. What next?